Which enzyme adds nucleotides in the 3' direction during DNA replication?

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Multiple Choice

Which enzyme adds nucleotides in the 3' direction during DNA replication?

Explanation:
DNA polymerases synthesize new DNA by adding nucleotides to the free 3' end of a growing strand, all in the 5' to 3' direction. This directionality is what drives replication: nucleotides are added one by one to the 3' end, extending the strand as the fork opens. Primase makes the RNA primer to give that starting 3'OH, but it does not continuously add DNA nucleotides. Helicase unwinds the double helix, and ligase joins Okazaki fragments afterward. So the enzyme that actually adds nucleotides in the 3' direction during DNA replication is DNA polymerase.

DNA polymerases synthesize new DNA by adding nucleotides to the free 3' end of a growing strand, all in the 5' to 3' direction. This directionality is what drives replication: nucleotides are added one by one to the 3' end, extending the strand as the fork opens. Primase makes the RNA primer to give that starting 3'OH, but it does not continuously add DNA nucleotides. Helicase unwinds the double helix, and ligase joins Okazaki fragments afterward. So the enzyme that actually adds nucleotides in the 3' direction during DNA replication is DNA polymerase.

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